ABSTRACT
The industrial utilization of enzymes requires the high yield of enzyme production for the synthesis of polymers by microorganisms. Therefore, it is necessary to optimize different production parameters of levansucrase in order to increase its industrial applications. Zymomonas mobilis KIBGE-IB14 was considered as a promising candidate for the large scale production of levan among wide range of microorganisms. The current investigation is aimed to optimize the production parameters of levansucrase by Z mobilis KIBGE-IB14 isolated from molasses. The results indicated that bacterial growth as well as enzyme production was greatly influenced by both physical and chemical conditions. It was revealed that high enzyme titers were achieved at 30°C with pH 6.5 after 24 hours of incubation in a modified medium. Moreover, the enzyme exhibited its induction in the presence of sucrose used as a substrate. Thus, the present study demonstrated that newly isolated Z mobilis KIBGE-IB14 can be used as a plausible producer of levansucrase for industrial applications
Subject(s)
Fermentation , Molasses , Sucrose , HexosyltransferasesABSTRACT
Increased bacterial resistance towards commonly used antibiotics has become a debated issue all over the world in a last few decades. Due to this, consumer demand towards natural anti-microbial agents is increasing day by day
Natural anti-microbial agents have gained enormous attention as an alternative therapeutic agent in pharmaceutical industry
Current study is an effort to explore and identify a bactericidal potential of various solvent extracts of Calotropis procera flower
Flowers of C. procera were extracted with hexane, butanol, ethyl acetate and aqua to evaluate the antibacterial activity by agar well diffusion method against the various human pathogens. The microorganisms used in this study includes Salmonella typhi, Escherichia coll [O157:H7], Micrococcus luteus KJBGE-IB20 [Gen Bank accession: JQ250612] and methicillin resistant Staphylococcus aureus [MRSA] KIBGE-IB23 [Gen Bank accession: KC465400]
Zones of inhibition were observed against all four pathogenic strains. Fraction soluble in hexane showed broad spectrum of inhibition against all the studied pathogens. However, fractions soluble in ethyl acetate inhibited the growth of E. coll, MRSA, and M. luteus. In case of butanol and aqueous extracts only growth of M. luteus was inhibited. Results revealed that the flower extracts of C. procera have a potential to be used as an antibacterial agent against these pathogenic organisms